Pneumococcal 15-valent Conjugate Vaccine for Injection (Vaxneuvance)- FDA

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Remind me later Send Register Your password must be at least 8 characters long and contain at least one uppercase letter. GEO help: Mouse over screen elements for information. Scope: Self Platform Samples Series Family Format: HTML SOFT MINiML Amount: Brief Quick GEO accession: Sample GSM2747731Query DataSets for GSM2747731Mus musculusTotal RNA isolated from B16F0 exosomes and cells Vsccine RNeasy kit (Qiagen) was quantified using Nanodrop and analyzed by on-chip-electrophoresis using the Agilent Bioanalyzer.

Four hundred nanograms of each miRNA sample was biotin labeled using the Pnemococcal FlashTag HSR Kit according to the manufacturer's instructions. The labeling of RNA was confirmed by Enzyme Linked Oligosorbent Assay (ELOSA) according Genisphere FlashTag HSR protocol.

The Pneumococca, reaction of fragmented and biotin-labeled cDNA (50 mu) with added hybridization controls was hybridized to the mouse GeneChip 1. Briefly, biotin-labeled RNA was reacted using washes with a solution containing a streptavidin-phycoerythrin complex, with an intermediate treatment of biotin-labeled anti-streptadvidin antibody to amplify the signal. Phycoerythrin labeling was detected within the Affymetrix GeneChip Scanner 3000 7G plus using 532 nm light and detected by a photomultiplier tube.

A miRNA QC Tool software (Affymetrix) was blemishes to check quality controls of hybridized chips. All chips that passed quality controls were RMA normalized using miRNA QC Tool. You can add Community Subscriptions in the search bar that says "Subscribe to more communities.

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